FLT1 antibody (Extracellular Domain)

Catalog No. ABIN115152

This anti-FLT1 antibody is a Mouse Monoclonal antibody detecting FLT1 in WB, IP, IF and EIA. Suitable for Human and Mouse.

Quick Overview for FLT1 antibody (Extracellular Domain) (ABIN115152)

Target: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This FLT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Clone: FLTEWF

Product Details anti-FLT1 Antibody

Binding Specificity: Extracellular Domain

Specificity: The monoclonal antibody will recognize recombinant and naturally occurring form of Human VEGFR-1/Flt-1.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Affinity Chromatography on Protein G

Immunogen: Recombinant Human soluble extracellular Flt-1 protein (D5) (Cat.-No DA3539)

Isotype: IgG1

Application Details

Application Notes: ELISA (1.5-8 μg/mL), Western blotting (1-10 μg/mL), Immunoprecipitation (1-5 μg/mL lysateor reaction volume).
Other applications not tested.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with distilled sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS pH 7.4 containing no preservative

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FLT1

Target: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Alternative Name: VEGFR-1 / Flt-1

Background: Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVE supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA.Synonyms: FLT, FLT1, FRT, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, VEGF Receptor 1, VEGFR1, Vascular endothelial growth factor receptor 1, Vascular permeability factor receptor

Gene ID: 2321

NCBI Accession: NP_001153392

UniProt: P17948

Pathways: RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals